The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection.
Identifieur interne : 000085 ( Main/Exploration ); précédent : 000084; suivant : 000086The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection.
Auteurs : José Javier Higuera [Espagne] ; José Garrido-Gala [Espagne] ; Ayman Lekhbou [Espagne] ; Isabel Arjona-Girona [Espagne] ; Francisco Amil-Ruiz [Espagne] ; José A. Mercado [Espagne] ; Fernando Pliego-Alfaro [Espagne] ; Juan Mu Oz-Blanco [Espagne] ; Carlos J. L Pez-Herrera [Espagne] ; José L. Caballero [Espagne]Source :
- Frontiers in plant science [ 1664-462X ] ; 2019.
Abstract
Strawberry (Fragaria ×ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in strawberry to Colletotrichum acutatum. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, Agrobacterium-mediated transient transformation was used both to silence and overexpress the FaWRKY1 gene in strawberry fruits (Fragaria ×ananassa cv. Primoris), which were later analyzed upon C. acutatum inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for FaWRKY1 overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where FaWRKY1 was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when FaWRKY1 overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus C. acutatum in strawberry fruit and contrast with the previous role described for this gene in Arabidopsis as positive regulator of resistance against the bacteria Pseudomonas syringae. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon C. acutatum infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen.
DOI: 10.3389/fpls.2019.00480
PubMed: 31057583
PubMed Central: PMC6482226
Affiliations:
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Mercado</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Pliego Alfaro, Fernando" sort="Pliego Alfaro, Fernando" uniqKey="Pliego Alfaro F" first="Fernando" last="Pliego-Alfaro">Fernando Pliego-Alfaro</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author><author><name sortKey="Mu Oz Blanco, Juan" sort="Mu Oz Blanco, Juan" uniqKey="Mu Oz Blanco J" first="Juan" last="Mu Oz-Blanco">Juan Mu Oz-Blanco</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="L Pez Herrera, Carlos J" sort="L Pez Herrera, Carlos J" uniqKey="L Pez Herrera C" first="Carlos J" last="L Pez-Herrera">Carlos J. L Pez-Herrera</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (CSIC), Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (CSIC), Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author><author><name sortKey="Caballero, Jose L" sort="Caballero, Jose L" uniqKey="Caballero J" first="José L" last="Caballero">José L. Caballero</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba</wicri:regionArea><wicri:noRegion>Córdoba</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Strawberry (<i>Fragaria</i> ×<i>ananassa</i>) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including <i>Colletotrichum</i> spp. So far, key factors regulating strawberry immune response remain unknown. The <i>FaWRKY1</i> gene has been previously proposed as an important element mediating defense responses in strawberry to <i>Colletotrichum acutatum</i>. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, <i>Agrobacterium</i>-mediated transient transformation was used both to silence and overexpress the <i>FaWRKY1</i> gene in strawberry fruits (<i>Fragaria</i> ×<i>ananassa</i> cv. Primoris), which were later analyzed upon <i>C. acutatum</i> inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for <i>FaWRKY1</i> overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where <i>FaWRKY1</i> was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when <i>FaWRKY1</i> overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus <i>C. acutatum</i> in strawberry fruit and contrast with the previous role described for this gene in <i>Arabidopsis</i> as positive regulator of resistance against the bacteria <i>Pseudomonas syringae</i>. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon <i>C. acutatum</i> infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31057583</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to <i>Colletotrichum acutatum</i> in Fruit Upon Infection.</ArticleTitle><Pagination><MedlinePgn>480</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2019.00480</ELocationID><Abstract><AbstractText>Strawberry (<i>Fragaria</i> ×<i>ananassa</i>) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including <i>Colletotrichum</i> spp. So far, key factors regulating strawberry immune response remain unknown. The <i>FaWRKY1</i> gene has been previously proposed as an important element mediating defense responses in strawberry to <i>Colletotrichum acutatum</i>. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, <i>Agrobacterium</i>-mediated transient transformation was used both to silence and overexpress the <i>FaWRKY1</i> gene in strawberry fruits (<i>Fragaria</i> ×<i>ananassa</i> cv. Primoris), which were later analyzed upon <i>C. acutatum</i> inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for <i>FaWRKY1</i> overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where <i>FaWRKY1</i> was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when <i>FaWRKY1</i> overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus <i>C. acutatum</i> in strawberry fruit and contrast with the previous role described for this gene in <i>Arabidopsis</i> as positive regulator of resistance against the bacteria <i>Pseudomonas syringae</i>. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon <i>C. acutatum</i> infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Higuera</LastName><ForeName>José Javier</ForeName><Initials>JJ</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Garrido-Gala</LastName><ForeName>José</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lekhbou</LastName><ForeName>Ayman</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arjona-Girona</LastName><ForeName>Isabel</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (CSIC), Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Amil-Ruiz</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Unidad de Bioinformática, Servicio Central de Apoyo a la Investigación (SCAI), Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mercado</LastName><ForeName>José A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pliego-Alfaro</LastName><ForeName>Fernando</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Departamento de Biologia Vegetal, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muñoz-Blanco</LastName><ForeName>Juan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Herrera</LastName><ForeName>Carlos J</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (CSIC), Córdoba, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Caballero</LastName><ForeName>José L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Córdoba, Córdoba, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium transformation</Keyword><Keyword MajorTopicYN="N">Colletotrichum acutatum</Keyword><Keyword MajorTopicYN="N">Fragaria × ananassa</Keyword><Keyword MajorTopicYN="N">WRKY transcription factor</Keyword><Keyword MajorTopicYN="N">strawberry fruit defense response</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>03</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>7</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31057583</ArticleId><ArticleId IdType="doi">10.3389/fpls.2019.00480</ArticleId><ArticleId 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sort="Higuera, Jose Javier" uniqKey="Higuera J" first="José Javier" last="Higuera">José Javier Higuera</name></noRegion><name sortKey="Amil Ruiz, Francisco" sort="Amil Ruiz, Francisco" uniqKey="Amil Ruiz F" first="Francisco" last="Amil-Ruiz">Francisco Amil-Ruiz</name><name sortKey="Arjona Girona, Isabel" sort="Arjona Girona, Isabel" uniqKey="Arjona Girona I" first="Isabel" last="Arjona-Girona">Isabel Arjona-Girona</name><name sortKey="Caballero, Jose L" sort="Caballero, Jose L" uniqKey="Caballero J" first="José L" last="Caballero">José L. Caballero</name><name sortKey="Garrido Gala, Jose" sort="Garrido Gala, Jose" uniqKey="Garrido Gala J" first="José" last="Garrido-Gala">José Garrido-Gala</name><name sortKey="L Pez Herrera, Carlos J" sort="L Pez Herrera, Carlos J" uniqKey="L Pez Herrera C" first="Carlos J" last="L Pez-Herrera">Carlos J. L Pez-Herrera</name><name sortKey="Lekhbou, Ayman" sort="Lekhbou, Ayman" uniqKey="Lekhbou A" first="Ayman" last="Lekhbou">Ayman Lekhbou</name><name sortKey="Mercado, Jose A" sort="Mercado, Jose A" uniqKey="Mercado J" first="José A" last="Mercado">José A. Mercado</name><name sortKey="Mu Oz Blanco, Juan" sort="Mu Oz Blanco, Juan" uniqKey="Mu Oz Blanco J" first="Juan" last="Mu Oz-Blanco">Juan Mu Oz-Blanco</name><name sortKey="Pliego Alfaro, Fernando" sort="Pliego Alfaro, Fernando" uniqKey="Pliego Alfaro F" first="Fernando" last="Pliego-Alfaro">Fernando Pliego-Alfaro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection.
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